ID 110881
Author
Hemdan, Dalia Ismaeil Ibrahim Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Hirasaka, Katsuya Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Nakao, Reiko Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kohno, Shohei Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kagawa, Sachiko Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Abe, Tomoki Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Harada-Sukeno, Akiko Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Okumura, Yuushi Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Nakaya, Yutaka Department of Nutritional Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Terao, Junji Department of Food Science, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nikawa, Takeshi Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
atrogenes
dexamethasone
mouse C2C12 cells
polyphenols
3D-clinorotation
Content Type
Journal Article
Description
Oxidative stress is a key factor in stimulating the expression of atrogenes, which are muscle atrophy-related ubiquitin ligases, in skeletal muscle, and it induces muscle atrophy during unloading. However, the effects of antioxidative nutrients on atrogene expression have not been demonstrated. We report on the inhibitory effects of polyphenols, such as epicatechin (EC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) and quercetin, on atrogene expression up-regulated by three dimensional (3D)-clinorotation or glucocorticoid. These treatments markedly elevated the expression of atrogenes, including atrogin-1 and MuRF-1, in mouse C2C12 myoblasts and myotubes. Interestingly, EC, ECg, EGCg and quercetin significantly decreased the expression of atrogin-1 and MuRF-1 up-regulated by 3D-clinorotation, whereas they hardly affected atrogene expression induced by dexamethasone. ERK signaling is a well known MAPK pathway to mediate oxidative stress. Therefore, we also investigated the effect of these polyphenols on phosphorylation of ERK in C2C12 myotubes. As expected, EC, ECg, EGCg, and quercetin significantly suppressed phosphorylation of ERK, corresponding to the up-regulation of atrogenes induced by 3D-clinorotation. These results suggest that antioxidative nutrients, such as catechins and quercetin, suppress atrogene expression in skeletal muscle cells, possibly through the inhibition of ERK signaling. Thus, catechins and quercetin may prevent unloading-mediated muscle atrophy.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
56
Issue
1-2
Start Page
26
End Page
32
Sort Key
26
Published Date
2009-02
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences