ID 110890
Author
Nonami, Kayo Tokushima University
Saitoh, Shohei Osaka University
Nishimura-Danjyobara, Yumiko Hiroshima University
Ishida, Shiro Tokushima Bunri University
Keywords
Chlorhexidine
Hydrogen peroxide
Cytotoxicity
Lymphocytes
Phosphatidylserine
Content Type
Journal Article
Description
Chlorhexidine (CHX) is an antibacterial agent used in various types of pharmaceutical products. Therefore, CHX is easily found around us. Owing to its positive charge, the electrochemical property of cell membranes was assumed to be a key point of cytotoxic action of CHX. Depolarization of membranes attenuated the cytotoxic action of CHX in rat thymic lymphocytes. CHX interfered with annexin V binding to membranes. Manipulations to induce exposure of phosphatidylserine on the outer membrane surface augmented the cytotoxic action of CHX, indicating that changes in the electrochemical property of membranes affected the cytotoxic action of CHX. Hence, CHX might kill cells physiologically undergoing apoptosis, resulting instead in necrotic cell death. However, the threshold CHX concentration in this in vitro study was slightly higher than blood CHX concentrations observed clinically. Therefore, these results may support the safety of CHX use although CHX possesses unique cytotoxic actions described in this study.
Journal Title
Environmental Toxicology and Pharmacology
ISSN
13826689
NCID
AA1107880X
Publisher
Elsevier
Volume
48
Start Page
17
End Page
21
Sort Key
17
Published Date
2016-10-01
Remark
© 2016 Elsevier B.V. All rights reserved.
© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry