ID 110891
Author
Keywords
G72
ab initio method
structure prediction
schizophrenia
D-amino acid oxidase
Content Type
Journal Article
Description
The G72 gene is one of the most susceptible genes to schizophrenia and is contained exclusively in the genomes of primates. The product of the G72 gene modulates the activity of D-amino acid oxidase (DAO) and is a small protein prone to aggregate, which hampers its structural studies. In addition, lack of a known structure of a homologue makes it difficult to use the homology modeling method for the prediction of the structure. Thus, we first developed a hybrid ab initio approach for small proteins prior to the prediction of the structure of G72. The approach uses three known ab initio algorithms. To evaluate the hybrid approach, we tested our prediction of the structure of the amino acid sequences whose structures were already solved and compared the predicted structures with the experimentally solved structures. Based on these comparisons, the average accuracy of our approach was calculated to be ∼5 Å. We then applied the approach to the sequence of G72 and successfully predicted the structures of the N- and C-terminal domains (ND and CD, respectively) of G72. The predicted structures of ND and CD were similar to membrane-bound proteins and adaptor proteins, respectively.
Journal Title
The Journal of Biochemistry
ISSN
17562651
0021924X
NCID
AA12096002
AA00694073
Publisher
Oxford University Press
Volume
161
Issue
2
Start Page
223
End Page
230
Sort Key
223
Published Date
2016-11-03
Remark
This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record, The Journal of Biochemistry (2017) Vol.161 Issue.2 p.223-230 is available online at: https://doi.org/10.1093/jb/mvw064.
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Institute of Advanced Medical Sciences