ID 111019
Author
Hoida, Ali Badr Tokushima University
Takahashi, Keiko Tokushima University
Keywords
Shiga toxin
seaweed
Ulva linza Linnaeus
rhamnan sulfate
Content Type
Journal Article
Description
We screened edible seaweeds in the ability to adsorb Shiga toxin (Stx) by an equilibrated dialysis method. Although water insoluble fractions of fourteen dry seaweeds did not adsorb Stx, most water soluble fractions were found to adsorb it to one degree or another. Among the seaweed tested, the extract of the Ulva linza Linnaeus [Enteromorpha linza (Linnaeus) J. Agardh] was found to well adsorb both Stx1 and Stx2. We purified the Stx-adsorbing substance from the U. linza extract by DEAE-Toyopearl column chromatography and gel filtration with HiPrep 16/60 Sephacryl S-300 HR column. The purified substance showed an average molecular mass of about 800 kDa by polyacrylamide gel electrophoresis. Analyses of its components indicated that the substance was a highly rhamnose-containing polysaccharide with sulfate esters of 18%. Apparent dissociation constants (Kd) of the polysaccharide to Stx1 and Stx2 were calculated to be 1.9 and 3.5 μM, respectively. To our knowledge, this is the first report on Stx-adsorbing dietary fibers.
Journal Title
European Food Research and Technology
ISSN
14382377
14382385
NCID
AA11622369
Publisher
Springer
Volume
243
Issue
12
Start Page
2147
End Page
2153
Published Date
2017-05-24
Remark
This is a post-peer-review, pre-copyedit version of an article published in European Food Research and Technology. The final authenticated version is available online at: http://doi.org/10.1007/s00217-017-2915-1
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry