ID 111187
Author
Morimoto, Masami The University of Tokushima
Takahashi, Masako Tokushima Breast Care Clinic
Honda, Junko Higashitokushima National Hospital
Sasa, Mitsunori Tokushima Breast Care Clinic
Keywords
Breast cancer
post-menopausal women
serum E2
high-sensitivity RIA
LC-MS/MS
Content Type
Journal Article
Description
Background : Serum E2 must be monitored for aromatase inhibitor (AI) therapy, but conventional assays lack sensitivity. Subjects/Methods : Forty amenorrheic breast cancer patients scheduled for AI treatment but requiring hormonological confirmation of their menopausal status were studied. Serum E2 data generated by high-sensitivity RIA and by LC-MS/MS were analyzed for correlation. Results : RIA gave a higher E2 value than LC-MS/MS in 62% of cases, but there was a significant positive correlation. Patients whose E2 levels by RIA were≥2.5 pg/mL higher than those by LC-MS/MS (RIA-H group) and all other patients (RIA-N group) were compared. Both groups showed strong correlations between the two assay methods. With both methods patients with a high BMI had significantly elevated E2. Multiple regression analysis used age, age at menarche, number of births and BMI as explanatory variables. Significant variables were the BMI with LC-MS/MS, and both BMI and age with RIA. The RIA-H and RIA-N groups showed no difference in regard to the BMI, whereas the age was significantly lower in the RIA-H group. Summary : Serum E2 levels determined for postmenopausal women by RIA and LC-MS/MS generally correlated well. High-sensitivity RIA is a potentially useful clinical assay, but it overestimated serum E2 in some women.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
63
Issue
3-4
Start Page
236
End Page
240
Sort Key
236
Published Date
2016-08
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
University Hospital
Medical Sciences