ID 111190
Author
Yukimasa, Nobuyasu Kagawa Prefectural University of Health Sciences
Sato, Shoichi Chiba Emergency Medical Center
Oboshi, Wataru Kagawa Prefectural University of Health Sciences
Watanabe, Toru Kagawa Prefectural University of Health Sciences
Uzawa, Ryuichi Uzawa Clinic
Keywords
hepatitis B vaccination
anti-HBs titers
cytokine gene polymorphisms
interleukin (IL)-10
tumor necrosis factor (TNF)-α
Content Type
Journal Article
Description
Hepatitis B (HB) vaccination is one of the most efficient tools to prevent the transmission of the virus. Considerable variability exists in HB vaccine responses, with 5-10% of healthy Japanese adults demonstrating no response following a standard vaccination. Recently, polymorphisms of immune-regulatory genes, such as cytokine genes, have been reported to influence the immune response to HB vaccine. The aim of this study was to investigate the underlying mechanisms of the genetic association between several cytokine gene polymorphisms and the immune response to HB vaccination in a Japanese population. One hundred and twenty three vaccinated young adults were classified according to the level of antibody-titer (anti-HBs). Single nucleotide polymorphism typing for IFN-γ(+874, 3’-UTR), IL-10 (−591, −819, −1082), and TNF-α(−308, −857), was accomplished using the PCR-RFLP or SSP-PCR method. The TNF-α(−857) CC type and the IL-10 (−1082) AG type were present more frequently in the low titer group than in the high titer group. The TNF-α(−857) CC type was found to be significantly associated with low response of serum anti-HBs. The anti-HBs antibody was not readily produced in the IL-10 (−1082) AG and TNF-α(−857) CC haplotype. Conversely, the antibody was readily produced in the IL-10 (−1082) AA and TNF-α(−857) CC haplotype, and the IL-10 (−1082) AA and TNF-α(−857) CT haplotype, suggesting a high likelihood of the IL-10 (−1082) AG type to be included in the low anti-HBs group, and high anti-HBs antibody production in those with the TNF-α(−857) CT type. These SNPs may produce ethnically-specific differences in the immune response to HB vaccine in the Japanese population.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
63
Issue
3-4
Start Page
256
End Page
261
Sort Key
256
Published Date
2016-08
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher