ID 111267
Author
Enomoto, Kei The University of Tokyo
Watanabe-Susaki, Kanako Advanced Industrial Science and Technology (AIST)
Kowno, Megumi The University of Tsukuba
Takada, Hitomi Advanced Industrial Science and Technology (AIST)
Yamanaka, Yuko Yokohama City University
Hirano, Hisashi Yokohama City University
Sugino, Hiromu Advanced Industrial Science and Technology (AIST)
Asashima, Makoto Advanced Industrial Science and Technology (AIST)|The University of Tokyo|Japan Science and Technology Agency (JST)
Kurisaki, Akira Advanced Industrial Science and Technology (AIST)|The University of Tsukuba
Keywords
Proteomics
ES cells
pluripotency
differentiation
proteins
Content Type
Journal Article
Description
Mammalian pluripotent stem cells possess properties of self-renewal and pluripotency. These abilities are maintained by the strict regulation of pluripotent stem cell-specific transcription factor network and unique properties of chromatin in the stem cells. Although these major signaling pathways robustly control the characteristics of stem cells, other regulatory factors, such as metabolic pathways, are also known to modulate stem cell proliferation and differentiation. In this study, we fractionated protein samples from mouse embryonic stem (ES) cells cultured with or without the leukemia inhibitory factor (LIF). Protein expression was quantified by 2-dimensional differential gel electrophoresis (2D-DIGE). In total, 44 proteins were identified as being differentially expressed in the pluripotent stem cells and the differentiated cells. Surprisingly, half of the identified proteins were the proteins localized in mitochondria, which supply cellular energy and regulate cell cycle, development, and cell death. Some of these identified proteins are involved in the metabolic function and the regulation of pluripotency. Further analysis of the identified proteins could provide new information for the manipulation of pluripotency in ES cells.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
62
Issue
3-4
Start Page
130
End Page
136
Sort Key
130
Published Date
2015-08
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Institute of Advanced Medical Sciences