ID 111333
Author
Inenaga, Kiyotoshi Kyushu Dental College
Yamada, Naoyuki Ajinomoto Co.
Yuji, Reiko Ajinomoto Co.
Kawai, Misako Ajinomoto Co.
Uneyama, Hisayuki Ajinomoto Co.
Ono, Kentaro Kyushu Dental College
Suzuki, Ei-ichiro Ajinomoto Co.
Torii, Kunio Ajinomoto Co.
Keywords
proteome
saliva
malnutrition
biomarker
Content Type
Journal Article
Description
Proteome analysis is a popular method to discover biomarkers for the prevention and diagnosis of diseases. Since saliva is a non-invasively available body fluid, gathering of saliva causes minimal harm to patients. Therefore, detection of proteins for the prevention and diagnosis from the saliva sample may be the preferred method, especially for children and elderly people. However, the abundance of salivary proteins and contaminant proteins from food and mouth bacteria obscure identification of proteins present in the saliva at low concentrations. To address this problem, we developed a shotgun proteomic method using two-dimensional nano-flow LC tandem mass spectrometry. We report here that our method is able to detect proteins quantitatively even in small sample volumes of saliva.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
56
Issue
Supplement
Start Page
224
End Page
227
Sort Key
224
Published Date
2009-12
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher