ID 111335
Author
Sakai, Takayoshi Osaka University
Keywords
salivary gland
branching morphogenesis
cleft formation
molecular analysis
T7-SAGE
Content Type
Journal Article
Description
Many organs such as the salivary glands, kidney and lung form multiple epithelial clefts during the process of branching morphogenesis in embryonic development. The salivary gland provides an excellent model for clarifying the mechanism of this phenomenon. Extracellular matrix molecules regulate the dynamics of this complex process of organ formation. Nevertheless, the mechanism of cleft formation is poorly understood. Here we describe that a combination of laser microdissection with T7-SAGE has been established as a method for gene discovery of candidate molecules that may be essential for early organ morphogenesis. We show a current approach that promises to improve our understanding of the early morphological changes. We are attempting to find novel regulators of branching morphogenesis. Fibronectin, lamininγ2, and TIMP-3 are differentially expressed in developing clefts or buds of submandibular glands. Fibronectinmediated crosstalks between integrin cell-to-matrix and cadherin cell-to-cell adhesion systems promote the formation of clefts. We have started studies to identify and characterize new molecules that may be important for cleft formation and fibronectin expression. We will introduce recent findings and future plans to identify new functional regulators. Progress in understanding the mechanisms of branching morphogenesis will provide novel approaches to future tissue engineering or regeneration of damaged organs.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
56
Issue
Supplement
Start Page
234
End Page
238
Sort Key
234
Published Date
2009-12
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher