ID 111339
Author
Hayashi, Toru Asahi University
Koyama, Noriko Asahi University
Gresik, Edward W The City University of New York
Kashimata, Masanori Asahi University
Keywords
microRNA
EGF
salivary gland
expression profile
Content Type
Journal Article
Description
Fetal murine submandibular salivary gland (SMG) is known as a model to study organogenesis including branching morphogenesis, which is a basic developmental process for formation of a wide variety of arborized organs. Branching morphogenesis is under the control of a complex network of regulatory proteins, such as the ErbB family of tyrosine kinase receptors, activated by members of the epidermal growth factor (EGF) family of ligands. Recent reports identify critical roles for micro RNAs (miRNAs) on many developmental processes through regulation of gene expression. We hypothesize that miRNAs regulating branching morphogenesis are expressed in fetal murine SMG and that expression of the miRNAs associated with branching morphogenesis is modulated in part by EGF. Using cloning methods, we obtained the expression profiles on miRNAs derived from fetal murine SMG under three different conditions : (1) native E13 SMGs (freshly isolated), (2) E13 SMGs cultured for 24 hours with no added EGF (controls), or (3) cultured with EGF. There were 44 known miRNAs and four novel miRNAs candidates in native SMG at E13. Comparing the three profiles revealed that several miRNAs were expressed specifically at each condition. These results suggested that these miRNAs were associated with regulating organogenesis, possibly including branching morphogenesis.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
56
Issue
Supplement
Start Page
250
End Page
252
Sort Key
250
Published Date
2009-12
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher