ID 111371
Author
Yamaguchi, Makoto Nagoya University
Ishiguro, Hiroshi Nagoya University
Steward, Martin University of Manchester
Sohma, Yoshiro Keio University
Yamamoto, Akiko Nagoya University
Shimouchi, Akito National Cardiovascular Center Research Institute
Kondo, Takaharu Nagoya University
Keywords
pancreatic duct cell
mathematical model
SLC26A6
Cl--HCO3- exchanger
Content Type
Journal Article
Description
Pancreatic duct cells secrete a HCO3--rich (~140 mM) fluid. Using a computer model of the pancreatic duct, Sohma, et al. have demonstrated that the activity of a Cl-/ HCO3- exchanger with a 1 : 1 stoichiometry at the apical membrane would have to be suppressed in order to achieve such a HCO3--rich secretion. Recently the apical exchanger in pancreatic ducts has been identified as SLC26A6 and this probably mediates most of Cl--dependent HCO3- secretion across the apical membrane. SLC26A6 is reported to mediate electrogenic Cl-/2HCO3- exchange when expressed in Xenopus oocytes. To assess the implications of this 1 : 2 stoichiometry for HCO3- secretion, we have reconstructed the Sohma model using MATLAB/Simulink. To do this we have formulated an expression for the turnover rate of Cl-/2HCO3- exchange using network thermodynamics and we have estimated the constants from published experimental data. Preliminary data suggest that the 1 : 2 stoichiometry of SLC26A6 would favor HCO3- secretion at higher concentrations.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
56
Issue
Supplement
Start Page
325
End Page
328
Sort Key
325
Published Date
2009-12
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher