ID 111459
Author
Ichikawa, Hideki Tokyo Dental College
Shibukawa, Yoshiyuki Tokyo Dental College
Sahara, Yoshinori Iwate Medical University
Tsumura, Maki Tokyo Dental College|Toho University
Qi, Bing Nihon University
Satoh, Keitaro Dokkyo Medical University
Narita, Takanori Nihon University
Hashimoto, Sadamitsu Tokyo Dental College
Momose, Yasunori Toho University
Tazaki, Masakazu Tokyo Dental College
Shimono, Masaki Tokyo Dental College
Sugiya, Hiroshi Nihon University
Keywords
AQP6
parotid acinar cells
ion transport
Content Type
Journal Article
Description
Salivary gland acinar cells secrete large amounts of water and electrolytes, where aquaporins (AQPs) are thought to be involved in the secretion. In the present study, we investigated expression/localization of AQP6, and the anion transporting properties of AQP6 in mouse parotid acinar cells. RT-PCR, western blotting and immunohistochemical analyses revealed expression of AQP6 in acinar cells, localized in apical membrane. Voltage ramp from -100 mV to +100 mV at a holding potential of -60 mV elicited outwardlyrectifying currents, in the presence of extracellular Cl- channel blockers and intracellular solution with 150 mM Cs+. These outward currents were increased when extracellular Cl- was replaced by Br-, NO3-, I-, or SCN-, accompanying a negative shift of reversal potentials. The outward current was enhanced by extracellular Hg2+. These results were consistent with the biophysical properties of transfected AQP6 oocytes or HEK cells, which indicate that the AQP6 channel is functionally expressed in parotid acinar cells, and suggest that AQP6 contributes to secretion of anions in parotid acinar cells.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
56
Issue
Supplement
Start Page
347
End Page
349
Sort Key
347
Published Date
2009-12
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher