ID 111479
Author
Ono, Kentaro Kyushu Dental College
Inagaki, Tomohiro Kyushu Dental College
Iida, Taichi Kyushu Dental College
Hosokawa, Ryuji Kyushu Dental College
Inenaga, Kiyotoshi Kyushu Dental College
Keywords
pilocarpine
cevimeline
parotid cells
Content Type
Others
Description
Previous reports suggested that there is no significant difference in the binding affinity of pilocarpine and cevimeline on muscarinic receptors (1). However, in vivo studies from our laboratory suggested that pilocarpine-induced salivation from the parotid gland is greater than that induced by cevimeline in rats (2, 3). Therefore, in the present study, sialogogue-induced intracellular Ca2+ mobilization was investigated in isolated parotid gland cells in vitro. Pilocarpine and cevimeline increased the intracellular Ca2+ concentration of parotid gland acinar and duct cells over 1 μM in a dose-dependent manner. Pilocarpine-induced responses were higher than cevimeline-induced responses. Ca2+ responses to both agents were completely blocked by 1 μM 4-DAMP, an M3 muscarinic receptor antagonist. In the absence of extracellular Ca2+, both sialogogues induced transient Ca2+ increase in parotid gland acinar cells. These results suggest that the sialogogues stimulate some common routes via the Ca2+ signaling in parotid gland acinar cells. We also report a significant difference of Ca2+ responses in concentration between pilocarpine and cevimeline in parotid gland acinar cells. The different Ca2+ responses between the sialogogues would explain the different saliva volumes from the parotid gland between them that we have observed in previous in vivo studies (2, 3).
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
56
Issue
Supplement
Start Page
375
End Page
375
Sort Key
375
Published Date
2009-12
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher