Tabuchi, Mari The University of Tokushima
Nagata, Hideya National Institute of Advanced Industrial Science and Technology (AIST)
Nomura, Masanori The University of Tokushima
Katsube, Sawako The University of Tokushima
Sawada, Akihiro The University of Tokushima
Joko, Akira The University of Tokushima
Hatta, Koji Nisshin Medical Instrument
Hagiwara, Naoto Taiyo Yuden Co., Ltd.
Kobayashi, Kazuyoshi Taiyo Yuden Co., Ltd.
Tomita, Fumiyo Taiyo Yuden Co., Ltd.
Miki, Satoko Taiyo Yuden Co., Ltd.
Arai, Katsuhiko Taiyo Yuden Co., Ltd.
Ishiguro, Takashi Taiyo Yuden Co., Ltd.
Baba, Yoshinobu National Institute of Advanced Industrial Science and Technology (AIST)|Nagoya University
The handing of the cells or tissues is essential for proteomics research or drug screening, where labor is not avoidable. The steps of cell wash, protein extraction, protein denaturing are complicated procedures in conventional method using centrifugation and pipetting in the laboratory. This is the bottle-neck for proteome research. To solve these problems, we propose to utilize the nanotechnology, which will improve the proteomics methodology. Utilizing the nanotechnology, we developed a novel microseparation system, where centrifugation and pipetting are needless. This system has a nanostructured microdevice, by which the cell handling, protein extraction, and antibody assay can be performed. Since cell transfer is needless, all cells are corrected without any loss during the cell-pretreatment procedures, which allowed high reproducibility and enabled the detection of low amount of protein expression. Utilizing the microdevice, we analyzed the stress induced proteins. We further succeeded the screening of food that was useful for immunity and found that an extraction from seaweed promoted the apoptosis of T-lymphoblastic cells. Here, we present an on-line microdevice for stress proteomics.
The Journal of Medical Investigation
Faculty of Medicine Tokushima University
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