ID 111619
Author
Yoshinaga, Sumiko The University of Tokushima
Nakahori, Yutaka The University of Tokushima
Yasuoka, Susumu The University of Tokushima
Keywords
trypsin-like enzyme
airway
fibrinogenolysis
sputum
chronic airway disease
Content Type
Journal Article
Description
Previously we isolated a new trypsin-like enzyme designated human airway trypsin-like protease (HAT) from human sputum. In this study,we examined in vitro whether HAT was related to the prevention of fibrin deposition in the airway lumen by cleaving fibrinogen. In mucoid sputum samples from patients with chronic airway diseases, the concentration of fibrinogen, as measured by ELISA, was in the range of 2-20μg/ml, and trypsin-like activity, as measured by spectrofluorometry was in the range of 10-50 milliunits (mU)/ml. We showed by gel filtration that the trypsin-like activity of mucoid sputum was mainly due to HAT. We examined the effects of HAT on human fibrinogen at pH 7.4 and 8.6. Fibrinogen was used at concentrations of 4-2,000μg/ml and HAT purified from sputum at concentrations of 0.6-10 mU/ml. As shown by SDS-polyacrylamide gel electrophoresis, HAT cleaved fibrinogen, especially its α-chain, regardless of the concentration of fibrinogen. Pretreatment of fibrinogen with HAT resulted in a decrease or complete loss of its thrombin-induced clotting capacity, depending on the duration of pretreatment with HAT and the concentration of HAT.
From these results we postulated that HAT may participate in the anticoagulation process within the airway, especially at the level of the mucous membrane, by cleaving fibrinogen transported from the blood stream.
Journal Title
The Journal of Medical Investigation
ISSN
13431420
NCID
AA11166929
Publisher
The University of Tokushima School of Medicine
Volume
45
Issue
1-4
Start Page
77
End Page
86
Sort Key
77
Published Date
1998-08
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher