ID 111642
Author
Ogushi, Fumitaka The University of Tokushima
Takahashi, Hiroko The University of Tokushima
Kawano, Tetsuya The University of Tokushima
Endo, Takeshi The University of Tokushima
Keywords
thrombin
fibroblast growth-stimulating factor
platelet-derived growth factor
pulmonary fibrosis
Content Type
Journal Article
Description
Thrombin is a multifunctional enzyme generated at sites of vascular injury, and is known to be increased in the lungs in some types of fibrotic lung disease. In this study, to determine whether thrombin is associated with fibroblast growth and pulmonary fibrosis in these disorders, we examined whether a growth factor for fibroblasts (platelet-derived growth factor, PDGF) was released by thrombin-stimulated alveolar macrophages (AM). The culture supernatants of rat AM stimulated with 1 or 10 U/ml of thrombin showed a significant increase in fibroblast growth-stimulating activity (FGA). Pretreatment of the AM supernatant with anti-PDGF-AA antibody significantly decreased the FGA, but pretreatment with anti-PDGF-BB antibody did not. The supernatants of AM stimulated with thrombin also increased the growth of fibroblasts from the lungs of rats with bleomycin-induced lung injury. These results indicate that thrombin stimulates AM to release PDGF-AA, which is responsible, at least in part, for fibroblast growth and the development of pulmonary fibrosis in some types of fibrotic lung disease.
Journal Title
The Journal of Medical Investigation
ISSN
13431420
NCID
AA11166929
Publisher
The University of Tokushima School of Medicine
Volume
44
Issue
1-2
Start Page
59
End Page
65
Sort Key
59
Published Date
1997-08
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
University Hospital
Medical Sciences