ID 111693
Title Alternative
RET MUTATIONS IN PHEOCHROMOCYTOMAS
Author
Kimura, Takehiko The University of Tokushima
Tanaka, Chisato The University of Tokushima KAKEN Search Researchers
Moritani, Maki The University of Tokushima
Iwahana, Hiroyuki The University of Tokushima
Keywords
RET proto-oncogene
Mutation
Codon 768
Exon 13
Pheochromocytoma
Content Type
Journal Article
Description
Sixteen sporadic pheochromocytomas, 3 pheochromocytomas in neurofibromatosis 1, and 4 pheochromocytomas in multiple endocrine neoplasia (MEN) 2A or 2B were screened for mutations at codon 768 of the RET proto-oncogene by AluI digestion of polymerase chain reaction (PCR) products and mutations in exon 13 by PCR-single strand conformation polymorphism (SSCP) analysis. Although mutations at codon 768 (GAG→GAC; Glu→Asp) of the RET proto-oncogene were recently reported to be found in 40% of sporadic medullary thyroid carcinomas (MTCs), the absence of missense mutations at codon 768 was confirmed both with PCR-restriction fragment length polymorphism (RFLP) and PCR-SSCP analysis in all examined cases of pheochromocytomas. These results suggest that mutations at codon 768 of the RET proto-oncogene do not represent a frequent mechanism of tumorigenesis for both sporadic and hereditary pheochromocytomas.
Journal Title
Endocrine Journal
ISSN
13484540
09188959
NCID
AA12020190
AA10901436
Publisher
The Japan Endocrine Society
Volume
43
Issue
1
Start Page
109
End Page
114
Published Date
1996
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences
University Hospital
Institute of Advanced Medical Sciences