ID 111860
Title Alternative
Induction of expressions of HLA class I and class II antigens by interferon on human renal cell carcinoma or bladder cancer cell lines
Author
Furukawa, Atsuko The University of Tokushima
Miyamoto, Tadayuki The University of Tokushima
Aki, Masashi The University of Tokushima
Kan, Masaharu The University of Tokushima
Keywords
renal cell carcinoma cell line
bladder cancer cell line
human leukocyte antigen (HLA)
interferon (IFN)
Content Type
Journal Article
Description
Interferon-induced expressions of HLA class I and class II (DR, DP, DQ) antigens on human renal cell carcinoma (RCC) cell lines and bladder cancer cell lines were analyzed by flowcytometry.
Class I antigen was present on all six different RCC cell lines and the expression was enhanced with IFN-γ treatment except one cell line, Caki-2. Although no RCC cell lines expressed class II antigens without IFN-γ treatment, the treatment induced the expression of DR antigen in all cell lines except Caki-2. DP antigen was induced on KPK-1 and KPK-13 with exposure to IFN-γ. On the other hand, bladder cancer cell lines studied showed surface expression of class I antigen and enhanced expression of class I antigen with IFN-γ treatment. However, IFN-γ didn't enhance the expression of class II antigens on any of those bladder cancer cell lines.
The minimum concentrations of IFN-γ to increase the expression of class I and DR andtigens on both KPK-13 and ACHN were 4 IU/ml and 100 IU/ml, respectively. The incubation time with IFN-γ to get the maximal expression of class I and DR antigens on KPK-13 were 36 hr and 48 hr, respectively. Meanwhile, although the expression of class I antigen on RCC cell lines was enhanced by IFN-α, IFN-α could not give any effect in class II antigens. Our results demonstrate the obvious effect of IFN-γ to enhance the expression of not only HLA class I antigen but also class II antigens on RCC cell lines.
Journal Title
Shikoku Acta Medica
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
52
Issue
2
Start Page
25
End Page
32
Sort Key
25
Published Date
1996-04-25
FullText File
language
jpn
TextVersion
Publisher
departments
Medical Sciences