Yoshida, Ichiro Shikoku Junior College
Ito, Chihiro Tokushima University
Matsuda, Shinya Tokushima University
Tsuji, Akihiko Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Yanaka, Noriyuki Hiroshima University
Yuasa, Keizo Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
microphthalmia-associated transcription factor (MITF)
To develop new whitening agents from natural products, we screened 80 compounds derived from crude drugs in Kampo medicine in a melanin synthesis inhibition assay using murine B16 melanoma cells. The screen revealed that treatment with alisol B, a triterpene from Alismatis rhizoma, significantly decreased both melanin content and cellular tyrosinase activity in B16 cells. However, alisol B did not directly inhibit mushroom tyrosinase activity in vitro. Therefore, we investigated the mechanism underlying the inhibitory effect of alisol B on melanogenesis. Alisol B suppressed mRNA induction of tyrosinase and its transcription factor, microphthalmia-associated transcription factor (MITF). Furthermore, alisol B reduced the phosphorylation of CREB and maintained the activation of ERK1/2. These results suggest that the reduction in melanin production by alisol B is due to the downregulation of MITF through the suppression of CREB and activation of ERK, and that alisol B may be useful as a new whitening agent.
Bioscience, Biotechnology, and Biochemistry
Japan Society for Bioscience, Biotechnology, and Agrochemistry|Taylor & Francis
This is an electronic version of an article published in Bioscience, Biotechnology, and Biochemistry 81, 3, 534-540, 2017. Bioscience, Biotechnology, and Biochemistry is available online at: www.tandfonline.com/10.1080/09168451.2016.1268042.
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|URL ( Publisher's Version )|
bbb_81_3_534.pdf 521 KB
Bioscience and Bioindustry