ID 111886
Author
Yoshida, Ichiro Shikoku Junior College
Ito, Chihiro Tokushima University
Matsuda, Shinya Tokushima University
Yanaka, Noriyuki Hiroshima University
Keywords
melanogenesis
alisol B
Kampo
tyrosinase
microphthalmia-associated transcription factor (MITF)
Content Type
Journal Article
Description
To develop new whitening agents from natural products, we screened 80 compounds derived from crude drugs in Kampo medicine in a melanin synthesis inhibition assay using murine B16 melanoma cells. The screen revealed that treatment with alisol B, a triterpene from Alismatis rhizoma, significantly decreased both melanin content and cellular tyrosinase activity in B16 cells. However, alisol B did not directly inhibit mushroom tyrosinase activity in vitro. Therefore, we investigated the mechanism underlying the inhibitory effect of alisol B on melanogenesis. Alisol B suppressed mRNA induction of tyrosinase and its transcription factor, microphthalmia-associated transcription factor (MITF). Furthermore, alisol B reduced the phosphorylation of CREB and maintained the activation of ERK1/2. These results suggest that the reduction in melanin production by alisol B is due to the downregulation of MITF through the suppression of CREB and activation of ERK, and that alisol B may be useful as a new whitening agent.
Journal Title
Bioscience, Biotechnology, and Biochemistry
ISSN
09168451
13476947
NCID
AA10824164
Publisher
Japan Society for Bioscience, Biotechnology, and Agrochemistry|Taylor & Francis
Volume
81
Issue
3
Start Page
534
End Page
540
Published Date
2017-01-04
Remark
This is an electronic version of an article published in Bioscience, Biotechnology, and Biochemistry 81, 3, 534-540, 2017. Bioscience, Biotechnology, and Biochemistry is available online at: www.tandfonline.com/10.1080/09168451.2016.1268042.
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DOI (Published Version)
URL ( Publisher's Version )
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language
eng
TextVersion
Author
departments
Bioscience and Bioindustry