ID 111976
Title Alternative
N-(3-oxododecanoyl)-L-homoserine-lactone, a quorum sensing molecule, causes a bell-shaped change in nonprotein thiol content in rat thymic lymphocytes : Its relation with oxidative stress and intracellular Zn2+
Author
Nishimura-Danjobara, Yumiko Tokushima University|Hiroshima University
Oyama, Keisuke Tokushima University|Osaka University
Takahashi, Keiko Tokushima University|Shikoku University
Keywords
Quorum sensing molecule
N-(3-oxododecanoyl)-L-homoserine-lactone
Lymphocytes
Intracellular Zn2+ levels
Oxidative stress
Glutathione
Content Type
Journal Article
Description
Cellular actions of N-(3-oxododecanoyl)-L-homoserine-lactone (ODHL), a quorum sensing molecule of bacteria, were studied on rat thymocytes using a flow cytometer with appropriate fluorescent dyes to elucidate the effects of ODHL on host cells. A bell-shaped concentration-response relation was observed in the ODHL-induced changes in cellular glutathione content ([GSH]i). ODHL concentration-dependently increased intracellular Zn2+ levels ([Zn2+]i) and cellular O2- content ([O2-]i). The bell-shaped relation induced by ODHL can be explained as follows: a low concentration of ODHL is expected to induce moderate oxidative stress that intracellularly releases Zn2+ by converting thiols to disulfides. A slight elevation of [Zn2+]i may increase the [GSH]i. On the other hand, it is likely that a high concentration of ODHL causes severe oxidative stress that further causes both the decrease in [GSH]i and the increase in [Zn2+]i. Excessive increase in [Zn2+]i may augment oxidative stress that further decreases the [GSH]i. Other notable actions induced by ODHL included the elevation of [Zn2+]i by Zn2+ influx and the increase in [GSH]i under Zn2+-free conditions. Therefore, it is suggested that ODHL elicits diverse actions on host cells.
Journal Title
Chemico-Biological Interactions
ISSN
00092797
NCID
AA0060252X
AA11523855
Publisher
Elsevier
Volume
280
Start Page
28
End Page
32
Published Date
2017-12-06
Rights
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry