ID 111990
Author
Kishida, Takumi Tokushima University
Funakoshi, Yurie Tokushima University
Fukuyama, Yuya Osaka City University
Honda, Sari Osaka City University
Keywords
4-vinylcatechol
lymphocytes
hydrogen peroxide
intracellular Zn2+
intracellular Ca2+
nonprotein thiol
cytotoxicity
Content Type
Journal Article
Description
4-Vinylcatechol (4VC) has been identified as an aroma compound in roasted foods, especially coffee. It is also a component in traditional herbal medicines. This compound may be subconsciously ingested through foods and herbs. Recent experimental evidence has shown that 4VC possesses an antioxidative action. However, the antioxidative action of 4VC at cellular levels is not well characterized. The effects of 4VC (0.1–100 μM) were examined on rat thymic lymphocytes without and with oxidative stress induced by 300 μM hydrogen peroxide (H2O2). Cell treatment with 100 μM 4VC alone for 4 hr significantly increased the population of dead cells. Thus, 4VC at 100 μM or above elicits cytotoxicity. However, 4VC at sublethal concentrations (1–10 μM) significantly attenuated the H2O2-induced increase in cell lethality in a concentration-dependent manner. While application of 10 μM 4VC slowed the process of cell death induced by H2O2, 4VC did not antagonize the H2O2-induced reduction of cellular nonprotein thiols. Although 4VC at 10 μM did not affect intracellular Ca2+ and Zn2+ levels, the agent potentiated the H2O2-induced increases in these levels. These actions of 10 μM 4VC are adverse to the cells under the oxidative stress. However, 10 μM 4VC partly attenuated the cell death induced by 100 nM A23187, a calcium ionophore. There are conflicting actions of 4VC at 1–100 μM on the cells under oxidative stress although the agent is used for an antioxidant. Thus, caution is required when using 4VC as a therapeutic agent.
Journal Title
Drug and Chemical Toxicology
ISSN
01480545
15256014
NCID
AA00161752
Publisher
Taylor & Francis
Published Date
2018-08-06
Remark
This is an Accepted Manuscript of an article published by Taylor & Francis in Drug and Chemical Toxicology on 06/08/2018, available online: http://www.tandfonline.com/10.1080/01480545.2018.1492604.
論文本文は2019-08-06以降公開予定
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
language
eng
TextVersion
その他
departments
Bioscience and Bioindustry