ID 112025
Author
Afroz, Sheuli Tokushima University
Yagi, Ayano Tokushima University
Fujikawa, Kouki Tokushima University
Rahman, M. Motiur Tokushima University
Morito, Katsuya Tokushima University
Watanabe, Shiro University of Toyama
Kiyokage, Emi Kawasaki Medical School|Kawasaki University of Medical Welfare
Toida, Kazunori Kawasaki Medical School|Osaka University
Keywords
Lysophosphatidic acid
Medicinal herbs
Indomethacin
Prostaglandin E2
Cell death
Cell proliferation
Content Type
Journal Article
Description
Lysophosphatidic acid (LPA) is a bioactive phospholipid that induces diverse biological responses. Recently, we found that LPA ameliorates NSAIDs-induced gastric ulcer in mice. Here, we quantified LPA in 21 medicinal herbs used for treatment of gastrointestinal (GI) disorders. We found that half of them contained LPA at relatively high levels (40–240 μg/g) compared to soybean seed powder (4.6 μg/g), which we previously identified as an LPA-rich food. The LPA in peony (Paeonia lactiflora) root powder is highly concentrated in the lipid fraction that ameliorates indomethacin-induced gastric ulcer in mice. Synthetic 18:1 LPA, peony root LPA and peony root lipid enhanced prostaglandin E2 production in a gastric cancer cell line, MKN74 cells that express LPA2 abundantly. These materials also prevented indomethacin-induced cell death and stimulated the proliferation of MKN74 cells. We found that LPA was present in stomach fluids at 2.4 μM, which is an effective LPA concentration for inducing a cellular response in vitro. These results indicated that LPA is one of the active components of medicinal herbs for the treatment of GI disorder and that orally administered LPA-rich herbs may augment the protective actions of endogenous LPA on gastric mucosa.
Journal Title
Prostaglandins & Other Lipid Mediators
ISSN
10988823
NCID
AA11221062
AA11532903
Publisher
Elsevier
Volume
135
Start Page
36
End Page
44
Published Date
2018-02-17
Rights
© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Pharmaceutical Sciences