ID 112079
Title Alternative
Studies on proliferation and differentiation of gastric epithelial cells using primary culture systems
Author
Keywords
primary cultures
gastric epithelial cells
proliferation and differentiation
pit cell lineage
Content Type
Journal Article
Description
Gastric fundic glands have a complex organization of several types of epithelial cells, including pit cells, parietal cells, neck cells, chief cells, and a variety of enteroendocrine cells. These functionally active cells come from multipotent stem cells that are found in the isthmus, and filiation and kinetics of these cells have been intensively studied in experimental animals. Transgenic mice have been introduced to study the mechanisms of cell lineage-specific and differentiation-dependent patterns of gene expression in the gastric units.
Primary cultures of gastric epithelial cells from rat, dog, rabbit, and guinea pig have been used to study interactions between distinct growth factors and gastric epithelial cells, and several growth factors, including epidermal growth factor (EGF). transforming growth factor-α, hepatocyte growth factor, insulin, and insulin-like growth factor 1, have been shown to stimulate proliferation of gastric epithelial cells. These primary cultures usually requires the presence of a high concentration of fetal calf serum, and cultured cells rapidly formed a monolayer within 2-3 days. A majority of the cells (90%) was identified as pit cells. We developed a complete serum-free culture of guinea pig gastric epithelial cells. The cells maintained in our culture conditions did not exhibit mitotic activity, and a great majority of the cells was in a pre-pit cell stage. EGF stimulated cell growth and then maturation into pit cells, suggesting that this culture system may be a excellent model to study the processes of maturation of a pit cell lineage.
Recently, gastric surface mucous cell lines (GSMO cells) from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene were established. A normal gastric epithelial cell line from rat gastric mucosa (RGM 1 cells) was also established. These cell lines may be useful for studying the proliferation and differentiation of a pit cell lineage.
Journal Title
Shikoku Acta Medica
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
54
Issue
2
Start Page
206
End Page
214
Sort Key
206
Published Date
1998-04-25
FullText File
language
jpn
TextVersion
Publisher
departments
Medical Sciences