ID 112153
Title Alternative
インフルエンザ感染を制御する気道内酵素
Enzymes and inhibitors in airway that regulate infection of influenza virus
Author
Murakami, Meiko The University of Tokushima
Towatari, Takae The University of Tokushima
Ohba, Kumiko The University of Tokushima
Keywords
influenza virus
tryptase clara
mini-plasmin
pulmonary surfactant
mucus protease inhibitor (MPI)
Content Type
Journal Article
Description
It has been proposed that the pathogenicity of the influenza and Sendai virus is primarily determined by host cellular proteases that activate viral infectivity. We isolated trypsin-type serin proteases from rat lungs, candidates for the processing proteases of viral envelope glycoproteins, such as tryptase Clara localized in the Clara cells of the bronchial epithelium and mini-plasmin. These enzymes specifically cleave the precursor of fusion glycoprotein HA of influenza virus at Arg325, and the Fo of Sendai virus at Arg116 in the consensus cleavage motif, Gln(Glu)-X-Arg, resulting in the induction of infectivity of these viruses. Proteolytic activation of viruses by these enzymes occurs extracellularly, probably on the surface and/or in the lumen of the respiratory tract. On the other hand, we isolated two compounds from human bronchial lavage, which inhibitor the activity of tryptase Clara. One was a mucus protease inhibitor and the other was a pulmonary surfactant.
These compounds inhibited multiple cycles of virus replication in vitro and in vivo, but did not themselves affect the hemagglutination and the infectivity of the virus. Administration of these compounds in the airway may be useful for preventing and treating infection with influenza virus and Sendai virus.
Journal Title
Shikoku Acta Medica
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
55
Issue
5
Start Page
174
End Page
179
Sort Key
174
Published Date
1999-10-25
EDB ID
FullText File
language
jpn
TextVersion
Publisher
departments
Institute of Advanced Medical Sciences