ID 112246
Title Alternative
Wild grape alleviates nasal symptoms.
Author
Islam, Rezwanul Tokushima University
Shaha, Aurpita Tokushima University
Nishida, Kohei Tokushima University
Yabumoto, Masami Medical Corporation Kinshukai
Ikeda, Hisashi Nab co., ltd.
Keywords
Allergic rhinitis
histamine H1 receptor
Interleukin-33
nuclear factor of activated T-cells
protein kinase Cδ
Content Type
Journal Article
Description
As expression level of allergic disease-sensitive genes are correlated with allergic symptom severity, suppression of these gene expressions could be good therapeutics. We have demonstrated that PKCδ signaling and NFAT signaling, involve in histamine H1 receptor (H1R) and IL-9 gene expressions, respectively, are responsible for the pathogenesis of allergic rhinitis. We explore anti-allergic compounds that suppress these signaling pathways and found that wild grape (WG) contains such compounds. Here, we investigated the effect of WG hot water extract (WGE) on the signaling pathways for PKCδ-mediated H1R and NFAT-mediated IL-9 gene expressions. WGE suppressed histamine/PMA-induced H1R gene up-regulation in HeLa cells. Toluene-2,4-diisocyanate (TDI)-induced H1R mRNA elevation in TDI-sensitized rats was also suppressed by WGE treatment. Treatment with WGE in combination with Awa-tea, suppresses NFAT signaling-mediated IL-9 gene, markedly alleviated nasal symptoms. Furthermore, WGE suppressed PMA-induced IL-33 gene up-regulation in Swiss 3T3 cells. Data suggest that combination of WGE, suppresses PKCδ signaling with Awa-tea, suppresses NFAT signaling would have distinct clinical and therapeutic advantages as a substitute for anti-allergic drugs. In addition, as the expression level of IL-33 mRNA was correlated with the blood eosinophils number in patients with pollinosis, WG could alleviate eosinophilic inflammation through the suppression of IL-33 gene expression.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA12022913
AA11166929
Publisher
Faculty of Medicine Tokushima University
Volume
65
Issue
3-4
Start Page
242
End Page
250
Sort Key
242
Published Date
2018-08
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences
Medical Sciences