ID 112314
Author
Liu, Jia Peking University|Tohoku University|Xinjiang Medical University
Saito, Kan Tohoku University
Maruya, Yuriko Tohoku University|Iwate Medical University
Nakamura, Takashi Tohoku University
Yamada, Aya Tohoku University
Fukumoto, Emiko Tohoku University
Ishikawa, Momoko Tohoku University
Miyazaki, Kanako Kyushu University
Yoshizaki, Keigo Kyushu University
Ge, Lihong Peking University
Fukumoto, Satoshi Tohoku University
Content Type
Journal Article
Description
Bone morphogenetic proteins (BMPs) regulate hard tissue formation, including bone and tooth. Growth differentiation factor 5 (GDF5), a known BMP, is expressed in cartilage and regulates chondrogenesis, and mutations have been shown to cause osteoarthritis. Notably, GDF5 is also expressed in periodontal ligament tissue; however, its role during tooth development is unclear. Here, we used cell culture and in vivo analyses to determine the role of GDF5 during tooth development. GDF5 and its associated BMP receptors are expressed at the protein and mRNA levels during postnatal tooth development, particularly at a stage associated with enamel formation. Furthermore, whereas BMP2 was observed to induce evidently the differentiation of enamel-forming ameloblasts, excess GDF5 induce mildly this differentiation. A mouse model harbouring a mutation in GDF5 (W408R) showed enhanced enamel formation in both the incisors and molars, but not in the tooth roots. Overexpression of the W408R GDF5 mutant protein was shown to induce BMP2-mediated mRNA expression of enamel matrix proteins and downstream phosphorylation of Smad1/5/8. These results suggest that mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-signalling.
Journal Title
Scientific Reports
ISSN
20452322
Publisher
Springer Nature
Volume
6
Start Page
23670
Published Date
2016-03-31
Remark
Supplementary Information : srep_6_23670_s1.pdf
Rights
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences