ID 112331
Title Alternative
Cellular Morphology Visualization to Probe Cell Differentiation
Author
Kamada, Rui Hokkaido University
Tano, Fumi Hokkaido University
Kudoh, Fuki Hokkaido University
Kimura, Nozomi Hokkaido University
Chuman, Yoshiro Hokkaido University
Osawa, Ayumi Hokkaido University
Tanino, Keiji Hokkaido University
Sakaguchi, Kazuyasu Hokkaido University
Content Type
Journal Article
Description
Nuclear and cytoplasmic morphological changes provide important information about cell differentiation processes, cell functions, and signal responses. There is a strong desire to develop a rapid and simple method for visualizing cytoplasmic and nuclear morphology. Here, we developed a novel and rapid method for probing cellular morphological changes of live cell differentiation process by a fluorescent probe, TAP-4PH, a 1,3a,6a-triazapentalene derivative. TAP-4PH showed high fluorescence in cytoplasmic area, and visualized cytoplasmic and nuclear morphological changes of live cells during differentiation. We demonstrated that TAP-4PH visualized dendritic axon and spine formation in neuronal differentiation, and nuclear structural changes during neutrophilic differentiation. We also showed that the utility of TAP-4PH for visualization of cytoplasmic and nuclear morphologies of various type of live cells. Our visualizing method has no toxicity and no influence on the cellular differentiation and function. The cell morphology can be rapidly observed after addition of TAP-4PH and can continue to be observed in the presence of TAP-4PH in cell culture medium. Moreover, TAP-4PH can be easily removed after observation by washing for subsequent biological assay. Taken together, these results demonstrate that our visualization method is a powerful tool to probe differentiation processes before subsequent biological assay in live cells.
Journal Title
PLOS ONE
ISSN
19326203
Publisher
PLOS
Volume
11
Issue
8
Start Page
e0160625
Published Date
2016-08-04
Rights
Copyright: © 2016 Kamada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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DOI (Published Version)
URL ( Publisher's Version )
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language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences