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ID 112386
Title Alternative
歯髄象牙芽細胞の自然免疫反応におけるNOD1の役割
Author
Washio, Ayako Kyushu Dental University
Kitamura, Chiaki Kyushu Dental University
Keywords
Odontoblasts
KN-3
NOD1
pulpitis
Content Type
Thesis or Dissertation
Description
Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis. Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts. In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA. The signal transduction pathway activated with NOD-specific ligand was assessed by blocking assay with specific inhibitors and reporter assay. In KN-3 cells, the expression level of NOD1 was stronger than that of NOD2 and the production of chemokines, such as CINC-1, CINC-2, CCL20, and MCP-1, was upregulated by stimulation with NOD1-specific ligand, but not with NOD2-specific ligand. CINC-2 and CCL20 production by stimulation with NOD1-specific ligand was reduced by p38 MAPK and AP-1 signaling inhibitors. Furthermore, the reporter assay demonstrated AP-1 activation in NOD1-specific ligand-stimulated KN-3 cells. These findings indicated that NOD1 expressed in odontoblasts functions to upregulate the chemokines expression via p38-AP-1 signaling pathway and suggested that NOD1 may play important roles in the initiation and progression of pulpitis.
Journal Title
BioMed Research International
ISSN
23146133
23146141
Publisher
Hindawi
Volume
2016
Start Page
9325436
Published Date
2016
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704261017.pdf
審査要旨:LID201704261018.pdf
論文本文:LID201704261019.pdf
本論文は,著者Yuki Hosokawaの学位論文として提出され,学位審査・授与の対象となっている。
Rights
Copyright ©2016 Yuki Hosokawa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.( https://creativecommons.org/licenses/by/4.0/ )
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3083号
Diploma Number
甲口第426号
Granted Date
2017-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
University Hospital