ID 112396
Title Alternative
ピエゾ型機械受容イオンチャネル1は間葉系幹細胞の分化運命決定の調節因子として機能する
Author
Miyazaki, Aya Tokushima University
Kawarabayashi, Keita Tokushima University
Yoshizaki, Keigo Kyushu University
Fukumoto, Satoshi Tohoku University
Keywords
PIEZO1
hydrostatic pressure
mesenchymal stem cells
osteoblast differentiation
BMP2
Content Type
Thesis or Dissertation
Description
The extracellular environment regulates the dynamic behaviors of cells. However, the effects of hydrostatic pressure (HP) on cell fate determination of mesenchymal stem cells (MSCs) are not clearly understood. Here, we established a cell culture chamber to control HP. Using this system, we found that the promotion of osteogenic differentiation by HP is depend on bone morphogenetic protein 2 (BMP2) expression regulated by Piezo type mechanosensitive ion channel component 1 (PIEZO1) in MSCs. The PIEZO1 was expressed and induced after HP loading in primary MSCs and MSC lines, UE7T-13 and SDP11. HP and Yoda1, an activator of PIEZO1, promoted BMP2 expression and osteoblast differentiation, whereas inhibits adipocyte differentiation. Conversely, PIEZO1 inhibition reduced osteoblast differentiation and BMP2 expression. Furthermore, Blocking of BMP2 function by noggin inhibits HP induced osteogenic maker genes expression. In addition, in an in vivo model of medaka with HP loading, HP promoted caudal fin ray development whereas inhibition of piezo1 using GsMTx4 suppressed its development. Thus, our results suggested that PIEZO1 is responsible for HP and could functions as a factor for cell fate determination of MSCs by regulating BMP2 expression.
Journal Title
Scientific Reports
ISSN
20452322
Publisher
Springer Nature
Volume
7
Start Page
17696
Published Date
2017-12-18
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Asuna Sugimotoの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© The Author(s) 2017
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3180号
Diploma Number
甲口第437号
Granted Date
2018-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Technical Support Department
University Hospital
Oral Sciences