ID 112451
Title Alternative
Effects of testosterone injection
Author
Yano, Kiyohito Tokushima University
Yanagihara, Rie Tokushima University
Mayila, Yiliyasi Tokushima University
Keywords
Testosterone
kisspeptin
RFRP
NKB
dynorphin
Content Type
Journal Article
Description
The effects of androgens on gonadotropin-releasing hormone (GnRH) secretion in females have not been fully established. To clarify the direct effects of androgens on hypothalamic reproductive factors, we evaluated the effects of chronic testosterone administration on hypothalamic GnRH regulatory factors in ovariectomized (OVX) female rats. Both testosterone and estradiol reduced the serum luteinizing hormone levels of OVX female rats, indicating that, as has been found for estrogen, testosterone suppresses GnRH secretion via negative feedback. Similarly, the administration of testosterone or estradiol suppressed the hypothalamic mRNA levels of kisspeptin and neurokinin B, both of which are positive regulators of GnRH, whereas it did not affect the hypothalamic mRNA levels of the kisspeptin receptor or neurokinin 3 receptor. On the contrary, the administration of testosterone, but not estradiol, suppressed the hypothalamic mRNA expression of prodynorphin, which is a negative regulator of GnRH. The administration of testosterone did not alter the rats’ serum estradiol levels, indicating that testosterone’s effects on hypothalamic factors might be induced by its androgenic activity. These findings suggest that as well as estrogen, androgens have negative feedback effects on GnRH in females and that the underlying mechanisms responsible for these effects are similar, but do not completely correspond, to the mechanisms underlying the effects of estrogen on GnRH.
Journal Title
Gynecological Endocrinology
ISSN
09513590
14730766
NCID
AA10839977
Publisher
Taylor & Francis
Volume
34
Issue
5
Start Page
437
End Page
441
Published Date
2017-11-29
Remark
This is an Accepted Manuscript of an article published by Taylor & Francis in Gynecological Endocrinology on 29/11/2017, available online: http://www.tandfonline.com/10.1080/09513590.2017.1409709.
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DOI (Published Version)
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language
eng
TextVersion
Author
departments
University Hospital
Medical Sciences