ID 112470
Author
Hisanaga, Ayumi Kagoshima University
Sakao, Kozue Kagoshima University
Hou, De-Xing Kagoshima University
Keywords
Cellular signaling
Direct binding
Inflammatory mediators
Molecular targets
Prenyl quercetin
Content Type
Journal Article
Description
Scope: 8-prenyl quercetin (PQ) is a typical prenylflavonoid distributed in plant foods and shows higher potential bioactivity than its parent quercetin (Q) although the mechanisms are not fully understood. This study aims to clarify the anti-inflammatory effects and molecular mechanisms of PQ in cell and animal models, compared to Q.
Methods and results: RAW264.7 cells were treated with PQ or Q to investigate the influence on the production of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and protein kinases by Western blotting. Nitric oxide (NO) and prostaglandin E2 (PGE2) were measured by the Griess method and ELISA, respectively. Cytokines were assayed by the multiplex technology. Mouse paw edema was induced by lipopolysaccharide (LPS). The results revealed that PQ had stronger inhibition on the productions of iNOS, COX-2, NO, PGE2, and 12 kinds of cytokines, than Q. PQ also showed in vivo anti-inflammatory effect by attenuating mouse paw edema. Molecular data revealed that PQ had no competitive binding to Toll-like receptor 4 (TLR4) with LPS, but directly targeted SEK1-JNK1/2 and MEK1-ERK1/2.
Conclusion: PQ as a potential inhibitor revealed anti-inflammatory effect in both cell and animal models at least by targeting SEK1-JNK1/2 and MEK1-ERK1/2.
Journal Title
Molecular Nutrition & Food Research
ISSN
16134125
16134133
NCID
AA12809751
AA11974242
Publisher
Wiley
Volume
60
Issue
5
Start Page
1020
End Page
1032
Published Date
2016-03-17
Remark
This is the peer reviewed version of the following article: Hisanaga, A. , Mukai, R. , Sakao, K. , Terao, J. and Hou, D. (2016), Anti‐inflammatory effects and molecular mechanisms of 8‐prenyl quercetin. Mol. Nutr. Food Res., 60: 1020-1032, which has been published in final form at https://doi.org/10.1002/mnfr.201500871. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry
Medical Sciences