TGF-β1 and IL-4 induce CCL11 production
Hosokawa, Yoshitaka Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hosokawa, Ikuko Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ozaki, Kazumi Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
periodontal ligament cells
Transforming growth factor (TGF)-β1 is a multifunctional cytokine, which can control certain functions of various kinds of cells. However, it is unclear whether TGF-β1 affects T-cell migration in periodontal lesions. The aim of this study was to examine the effects of TGF-β1 on the production of C-C chemokine ligand (CCL)11, which is a T-helper 2-type chemokine, in human periodontal ligament cells (HPDLC). Interleukin (IL)-4 induced CCL11 production, but TGF-β1 did not, in HPDLC. However, TGF-β1 enhanced CCL11 production in IL-4-stimulated HPDLC. Western blot analysis showed that the signal transducer and activator of transcription 6 (STAT6) pathway was highly activated in HPDLC that had been stimulated with both IL-4 and TGF-β1. Mitogen-activated protein kinase activation did not differ between the HPDLC treated with a combination of IL-4 and TGF-β1 and those treated with IL-4 or TGF-β1 alone. Moreover, a STAT6 inhibitor significantly inhibited CCL11 production in HPDLC that had been stimulated with IL-4 and TGF-β1. The current study clearly demonstrated that TGF-β1 enhanced IL-4-induced CCL11 production in HPDLC. The STAT6 pathway is important for CCL11 production in IL-4- and TGF-β1-treated HPDLC.
Cell Biology International
International Federation for Cell Biology|Wiley
This is the peer reviewed version of the following article: Hosokawa, Y. , Hosokawa, I. , Ozaki, K. and Matsuo, T. (2018), Transforming growth factor‐β1 increases C‐C chemokine ligand 11 production in interleukin 4‐stimulated human periodontal ligament cells. Cell Biol Int, 42: 1395-1400, which has been published in final form at https://doi.org/10.1002/cbin.11030.
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© 2018 International Federation for Cell Biology
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