ID 113011
Author
Miyamoto, Koji NOF Corporation
Hirota, Katsuhiko Tokushima University|Kochi Gakuen College KAKEN Search Researchers
Nakae, Hiromi Tokushima University|Tokushima Bunri University KAKEN Search Researchers
Tanaka, Saya Tokushima University
Keywords
2-Methacryloyloxyethyl phosphorylcholine
Clinical study
Oral infection
Oral bacteria
Fusobacterium nucleatum
Content Type
Journal Article
Description
Objectives The biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymers, which mimic a biomembrane, reduce protein adsorption and bacterial adhesion and inhibit cell attachment. The aim of this study is to clarify whether MPC-polymer can suppress the bacterial adherence in oral cavity by a crossover design. We also investigated the number of Fusobacterium nucleatum, which is the key bacterium forming dental plaque, in clinical samples.
Materials and methods This study was a randomized, placebo-controlled, single-blind, crossover study, with two treatment periods separated by a 2-week washout period. We conducted clinical trial with 20 healthy subjects to evaluate the effect of 5% MPC-polymer mouthwash after 5 h on oral microflora. PBS was used as a control. The bacterial number in the gargling sample before and after intervention was counted by an electronic bacterial counter and a culture method. DNA amounts of total bacteria and F. nucleatum were examined by q-PCR.
Results The numbers of total bacteria and oral streptcocci after 5 h of 5% MPC-polymer treatment significantly decreased, compared to the control group. Moreover, the DNA amounts of total bacteria and F. nucleatum significantly decreased by 5% MPC-polymer mouthwash.
Conclusions We suggest that MPC-polymer coating in the oral cavity may suppress the oral bacterial adherence.
Clinical relevance MPC-polymer can be a potent compound for the control of oral microflora to prevent oral infection.
Journal Title
Clinical Oral Investigations
ISSN
14326981
14363771
NCID
AA11119758
AA11619637
Publisher
Springer Berlin Heidelberg|German Society of Dental, Oral and Craniomandibular Sciences|European Federation of Conservative Dentistry
Volume
23
Issue
2
Start Page
739
End Page
746
Published Date
2018-05-16
Rights
© The Author(s) 2018 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences
University Hospital