ID 113064
Author
Imura, Naohiro Tokushima University
Ae, Masamichi Tokushima University
Hoshino, Rin Tokushima University
Abe, Masami Tokushima University
Yamamuro, Tatsuya Tokushima University
Oyama, Keisuke Sakai City Medical Center
Keywords
Azoxystrobin
Lymphocytes
Membrane potential
Intracellular calcium
Content Type
Journal Article
Description
Azoxystrobin, a broad-spectrum fungicide, has been increasingly used in the agricultural industry. In Japan in 2018, azoxystrobin at five times the normal limit was detected in a shipment of Australian barley that had been used in food products. Therefore, the effects of azoxystrobin need to be carefully examined to predict potential adverse reactions in humans. In this study, the effects of azoxystrobin on the membrane potential and intracellular Ca2+ levels of thymocytes have been photochemically examined using flow cytometry. Azoxystrobin hyperpolarized plasma membrane potential. This hyperpolarization appeared to be due to the activation of Ca2+-dependent K+ channels, as both the removal of extracellular Ca2+ and addition of charybdotoxin attenuated the observed hyperpolarization. In the presence of quinine, an anti-malarial drug that blocks Ca2+-dependent K+ channels, azoxystrobin depolarized the membranes instead. Azoxystrobin increased intracellular Ca2+ levels in a concentration-dependent manner through the influx of extracellular Ca2+ and intracellular release of Ca2+, as confirmed by reduction in azoxystrobin-induced response in the absence of extracellular Ca2+. It appears likely that azoxystrobin at micromolar concentrations modifies membrane ion permeability in thymocytes. Since changes in membrane potential and intracellular Ca2+ levels occur during typical physiological lymphocyte responses, azoxystrobin may disturb lymphocyte function.
Journal Title
Chemico-Biological Interactions
ISSN
00092797
NCID
AA0060252X
AA11523855
Publisher
Elsevier
Volume
300
Start Page
35
End Page
39
Published Date
2019-01-07
Remark
論文本文は2020-01-07以降公開予定
Rights
© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
language
eng
TextVersion
その他
departments
Bioscience and Bioindustry