ID | 114071 |
Author |
Hama, Susumu
Kyoto Pharmaceutical University
Okamura, Yuriko
Kyoto Pharmaceutical University
Kamei, Kazuho
Kyoto Pharmaceutical University
Nagao, Saki
Kyoto Pharmaceutical University
Hayashi, Mari
Kyoto Pharmaceutical University
Maeda, Shizuka
Kyoto Pharmaceutical University
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Keywords | Tocopheryl succinate
Angiogenesis
Angiopoietin
Vascular stabilization
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Content Type |
Journal Article
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Description | α-Tocopheryl succinate (TS) is a tocopherol derivative and has multifaceted anti-cancer effects; TS not only causes cancer cell-specific apoptosis but also inhibits tumor angiogenesis. Although TS has the potential to be used as a well-tolerated anti-angiogenic drug, it is still unclear which step of the angiogenic process is inhibited by TS. Here, we show that TS inhibits the expression of angiopoietin (Ang)-2, which induces destabilization of vascular structure in the initial steps of the angiogenic process. In mouse melanoma cells, TS treatment decreased mRNA and extracellular protein levels of Ang-2; however, the mRNA level of Ang-1, which stabilizes the vascular structure, remained unchanged. Furthermore, aorta ring and Matrigel plug angiogenesis assays indicated that the conditioned medium from TS-treated cells (CM-TS) inhibited neovascularization and blood leakage from the existing blood vessels, respectively. Following immunohistochemical staining of the vessels treated with CM-TS, imaging studies showed that the vascular endothelial cells were highly packed with pericytes. In conclusion, we found that TS inhibits Ang-2 expression and, consequently, stabilizes the vascular structure during the initial step of tumor angiogenesis.
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Journal Title |
Biochemical and Biophysical Research Communications
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ISSN | 0006291X
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NCID | AA00564395
AA11542044
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Publisher | Elsevier
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Volume | 521
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Issue | 4
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Start Page | 947
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End Page | 951
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Published Date | 2019-11-11
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Rights | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
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language |
eng
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TextVersion |
Author
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departments |
Pharmaceutical Sciences
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