ID | 114213 |
Title Alternative | 骨-歯根膜線維の複合組織形成による三次元的な歯周組織再生技術の開発
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Author |
Raju, Resmi
Tokushima University
Oshima, Masamitsu
Tokushima University
Tokushima University Educator and Researcher Directory
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Inoue, Miho
Tokushima University
Tokushima University Educator and Researcher Directory
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Morita, Tsuyoshi
Tokushima University
Tokushima University Educator and Researcher Directory
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Huijiao, Yan
Tokushima University
Waskitho, Arief
Tokushima University
Baba, Otto
Tokushima University
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Inoue, Masahisa
Tokushima Bunri University
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Keywords | Cell sheet
Periodontal tissue
Regeneration
Periodontal ligament cell
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Content Type |
Thesis or Dissertation
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Description | Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure.
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Journal Title |
Scientific Reports
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ISSN | 20452322
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Publisher | Springer Nature
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Volume | 10
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Start Page | 1656
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Published Date | 2020-02-03
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Remark | 内容要旨・審査要旨・論文本文の公開:
内容要旨 : k3381_abstract.pdf 審査要旨 : k3381_review.pdf 論文本文 : k3381_fulltext.pdf (著者最終稿) 論文本文 : srep_10_1656.pdf (雑誌掲載版) 本論文は, 著者Resmi Rajuの学位論文として提出され, 学位審査・授与の対象となっている。 |
Rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3381号
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Diploma Number | 甲口第460号
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Granted Date | 2020-03-23
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Degree Name |
Doctor of Dental Science
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Grantor |
Tokushima University
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departments |
Oral Sciences
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