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ID 114781
Furuya, Masayuki Osaka University
Kikuta, Junichi Osaka University
Seno, Shigeto Osaka University
Maeda, Hiroki Osaka University
Shirazaki, Mai Osaka University
Uenaka, Maki Osaka University
Mizuno, Hiroki Osaka University
Iwamoto, Yoriko Osaka University
Morimoto, Akito Osaka University
Hashimoto, Kunihiko Osaka University
Ito, Takeshi Asahi Kasei Pharma Corporation
Isogai, Yukihiro Asahi Kasei Pharma Corporation
Kashii, Masafumi Osaka University
Kaito, Takashi Osaka University
Ohba, Shinsuke The University of Tokyo
Chung, Ung-il The University of Tokyo
Lichtler, Alexander C. University of Connecticut
Kikuchi, Kazuya Osaka University
Matsuda, Hideo Osaka University
Yoshikawa, Hideki Osaka University
Ishii, Masaru Osaka University
Content Type
Journal Article
Bone homeostasis is regulated by communication between bone-forming mature osteoblasts (mOBs) and bone-resorptive mature osteoclasts (mOCs). However, the spatial–temporal relationship and mode of interaction in vivo remain elusive. Here we show, by using an intravital imaging technique, that mOB and mOC functions are regulated via direct cell–cell contact between these cell types. The mOBs and mOCs mainly occupy discrete territories in the steady state, although direct cell–cell contact is detected in spatiotemporally limited areas. In addition, a pH-sensing fluorescence probe reveals that mOCs secrete protons for bone resorption when they are not in contact with mOBs, whereas mOCs contacting mOBs are non-resorptive, suggesting that mOBs can inhibit bone resorption by direct contact. Intermittent administration of parathyroid hormone causes bone anabolic effects, which lead to a mixed distribution of mOBs and mOCs, and increase cell–cell contact. This study reveals spatiotemporal intercellular interactions between mOBs and mOCs affecting bone homeostasis in vivo.
Journal Title
Nature Communications
Springer Nature
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Published Date
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© The Author(s) 2018
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Institute of Advanced Medical Sciences