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ID 114784
Harada, Akihito Kyushu University
Maehara, Kazumitsu Kyushu University
Ono, Yusuke Nagasaki University
Taguchi, Hiroyuki Waseda University
Yoshioka, Kiyoshi Nagasaki University
Kitajima, Yasuo Nagasaki University
Xie, Yan Waseda University
Sato, Yuko Tokyo Institute of Technology
Iwasaki, Takeshi Kyushu University
Nogami, Jumpei Kyushu University
Okada, Seiji Kyushu University
Komatsu, Tetsuro Kyushu University
Semba, Yuichiro Kyushu University
Kimura, Hiroshi Tokyo Institute of Technology
Kurumizaka, Hitoshi Waseda University
Ohkawa, Yasuyuki Kyushu University
Content Type
Journal Article
Regulation of gene expression requires selective incorporation of histone H3 variant H3.3 into chromatin. Histone H3.3 has several subsidiary variants but their functions are unclear. Here we characterize the function of histone H3.3 sub-variant, H3mm7, which is expressed in skeletal muscle satellite cells. H3mm7 knockout mice demonstrate an essential role of H3mm7 in skeletal muscle regeneration. Chromatin analysis reveals that H3mm7 facilitates transcription by forming an open chromatin structure around promoter regions including those of myogenic genes. The crystal structure of the nucleosome containing H3mm7 reveals that, unlike the S57 residue of other H3 proteins, the H3mm7-specific A57 residue cannot form a hydrogen bond with the R40 residue of the cognate H4 molecule. Consequently, the H3mm7 nucleosome is unstable in vitro and exhibited higher mobility in vivo compared with the H3.3 nucleosome. We conclude that the unstable H3mm7 nucleosome may be required for proper skeletal muscle differentiation.
Journal Title
Nature Communications
Springer Nature
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© The Author(s) 2018
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Institute of Advanced Medical Sciences