number of access : ?
number of downloads : ?
ID 115503
Title Alternative
Characterization of α-gustducin
Author
Udagawa, Haruhide National Center for Global Health and Medicine|Kyorin University
Hiramoto, Masaki Tokyo Medical University
Kawaguchi, Miho National Center for Global Health and Medicine
Ohara‐Imaizumi, Mica Kyorin University
Nammo, Takao National Center for Global Health and Medicine
Nishimura, Wataru International University of Health and Welfare|Jichi Medical University
Yasuda, Kazuki National Center for Global Health and Medicine|Kyorin University
Keywords
α-Gustducin
Insulin secretion
Taste receptor
Content Type
Journal Article
Description
Aims/Introduction: Taste receptors, T1rs and T2rs, and the taste‐selective G‐protein, α‐gustducin, are expressed outside the taste‐sensing system, such as enteroendocrine L cells. Here, we examined whether α‐gustducin also affects nutrition sensing and insulin secretion by pancreatic β‐cells.
Materials and Methods: The expression of α‐gustducin and taste receptors was evaluated in β‐cell lines, and in rat and mouse islets either by quantitative polymerase chain reaction or fluorescence immunostaining. The effects of α‐gustducin knockdown on insulin secretion and on cyclic adenosine monophosphate and intracellular Ca2+ levels in rat INS‐1 cells were estimated. Sucralose (taste receptor agonist)‐induced insulin secretion was investigated in INS‐1 cells with α‐gustducin suppression and in islets from mouse disease models.
Results: The expression of Tas1r3 and α‐gustducin was confirmed in β‐cell lines and pancreatic islets. Basal levels of cyclic adenosine monophosphate, intracellular calcium and insulin secretion were significantly enhanced with α‐gustducin knockdown in INS‐1 cells. The expression of α‐gustducin was decreased in high‐fat diet‐fed mice and in diabetic db/db mice. Sucralose‐induced insulin secretion was not attenuated in INS‐1 cells with α‐gustducin knockdown or in mouse islets with decreased expression of α‐gustducin.
Conclusions: α‐Gustducin is involved in the regulation of cyclic adenosine monophosphate, intracellular calcium levels and insulin secretion in pancreatic β‐cells in a manner independent of taste receptor signaling. α‐Gustducin might play a novel role in β‐cell physiology and the development of type 2 diabetes.
Journal Title
Journal of Diabetes Investigation
ISSN
20401124
NCID
AA12488319
Publisher
Asian Association for the Study of Diabetes|John Wiley & Sons
Volume
11
Issue
4
Start Page
814
End Page
822
Published Date
2020-01-20
Rights
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License(https://creativecommons.org/licenses/by-nc/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences