ID | 115685 |
Title Alternative | Maackiain Suppresses H1R and IL-4 Gene Transcriptions
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Author |
Nariai, Yuki
Tokushima University
Kato, Shuhei
Tokushima University
Nakano, Tomohiro
Tokushima University
Kanayama, Tomoyo
Tokushima University
Kashiwada, Yoshiki
Tokushima University
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Takaishi, Yoshihisa
Tokushima University
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Kitamura, Yoshiaki
Tokushima University
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Keywords | Allergic disease sensitive gene
histamine H1 receptor gene
IL-4 gene
Kujin
maackiain
PKCδ
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Content Type |
Journal Article
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Description | Kujin contains antiallergic compounds that inhibit upregulation of histamine H1 receptor (H1R) and interleukin (IL)‐4 gene expression. However, the underlying mechanism remains unknown. We sought to identify a Kujin‐derived antiallergic compound and investigate its mechanism of action. The H1R and IL‐4 mRNA levels were determined by real‐time quantitative RT‐PCR. To investigate the effects of maackiain in vivo, toluene‐2,4‐diisocyanate (TDI)‐sensitized rats were used as a nasal hypersensitivity animal model. We identified (−)‐maackiain as the responsible component. Synthetic maackiain showed stereoselectivity for the suppression of IL‐4 gene expression but not for H1R gene expression, suggesting distinct target proteins for transcriptional signaling. (−)‐Maackiain inhibited of PKCδ translocation to the Golgi and phosphorylation of Tyr311 on PKCδ, which led to the suppression of H1R gene transcription. However, (−)‐maackiain did not show any antioxidant activity or inhibition of PKCδ enzymatic activity per se. Pretreatment with maackiain alleviated nasal symptoms and suppressed TDI‐induced upregulations of H1R and IL‐4 gene expressions in TDI‐sensitized rats. These data suggest that (−)‐maackiain is a novel antiallergic compound that alleviates nasal symptoms in TDI‐sensitized allergy model rats through the inhibition of H1R and IL‐4 gene expression. The molecular mechanism underlying its suppressive effect for H1R gene expression is mediated by the inhibition of PKCδ activation.
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Journal Title |
Pharmacology Research & Perspectives
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ISSN | 20521707
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Publisher | John Wiley & Sons|British Pharmacological Society|American Society for Pharmacology and Experimental Therapeutics
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Volume | 3
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Issue | 5
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Start Page | e00166
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Published Date | 2015-08-10
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Rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License(https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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language |
eng
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Publisher
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departments |
Pharmaceutical Sciences
Medical Sciences
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