Hasan, Mahadi Kyoto Pharmaceutical University
Tarashima, Noriko Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Fujikawa, Koki Tokushima University
Ohgita, Takashi Kyoto Pharmaceutical University
Hama, Susumu Kyoto Pharmaceutical University
Tanaka, Tamotsu Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Minakawa, Noriaki Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
faint electric treatment
An intelligent shRNA expression device (iRed) contains the minimum essential components needed for shRNA production in cells, and could be a novel tool to regulate target genes. However, general delivery carriers consisting of cationic polymers/lipids could impede function of a newly generated shRNA via electrostatic interaction in the cytoplasm. Recently, we found that faint electric treatment (fET) of cells enhanced delivery of siRNA and functional nucleic acids into the cytoplasm in the absence of delivery carriers. Here, we examined fET of cells stably expressing luciferase in the presence of iRed encoding anti-luciferase shRNA. Transfection of lipofectamine 2000 (LFN)/iRed lipoplexes showed an RNAi effect, but fET-mediated iRed transfection did not, likely because of the endosomal localization of iRed after delivery. However, fET in the presence of lysosomotropic agent chloroquine significantly improved the RNAi effect of iRed/fET to levels that were higher than those for the LFN/iRed lipoplexes. Furthermore, the amount of lipid droplets in adipocytes significantly decreased following fET with iRed against resistin in the presence of chloroquine. Thus, iRed could be a useful tool to regulate target genes following fET-mediated cytoplasmic delivery with endosomal escape devices.
Science and Technology of Advanced Materials
Taylor & Francis|National Institute for Materials Science
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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stam_17_1_554.pdf 2.66 MB