ID | 116295 |
Author |
Nakagawa, Kasumi
Tokushima University
Takeuchi, Michiki
Kyoto University
Kikuchi, Mayu
Kyoto University
Kiyofuji, Suzuna
Kyoto University
Kugo, Masami
Kyoto University
Sakamoto, Takaiku
Tokushima University
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Kano, Kenji
Kyoto University
Ogawa, Jun
Kyoto University
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Keywords | Indigo Reduction Mechanism
Acetaldehyde
Bioelectrocatalysis
Mediator
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Content Type |
Journal Article
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Description | Indigo is one of the oldest natural blue dyes. Microorganisms and their enzymatic activities are deeply involved in the traditional indigo staining procedure. To elucidate the mechanism of the microbial indigo reduction, we directly performed cyclic voltammetry on alkaline fermenting dye suspensions. A pair of characteristic redox peaks of leuco-indigo was observed in a supernatant fluid of the fermenting dye suspension. On the other hand, it was found that the indigo/leuco-indigo redox couple mediated two-way microbially catalyzed oxidation and reduction in a sediment-rich suspension of the fermenting suspension. Acetaldehyde was supposed to be the electron donor and acceptor of the catalytic reactions. In order to verify the bioelectrocatalytic reaction, we isolated indigo-reducing bacterium K2-3′ from the fermenting suspension, and the two-way bioelectrocatalysis was successfully restaged in a model system containing K2-3′ and methyl viologen (as a soluble mediator instead of indigo) as well as acetaldehyde at pH 10.
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Journal Title |
Electrochemistry
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ISSN | 21862451
13443542
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Publisher | The Electrochemical Society of Japan
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Volume | 89
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Issue | 1
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Start Page | 25
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End Page | 30
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Published Date | 2021-01-05
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Rights | This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License (CC BY, http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse of the work in any medium provided the original work is properly cited. [DOI: 10.5796/electrochemistry.20-00123].
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language |
eng
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TextVersion |
Publisher
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departments |
Bioscience and Bioindustry
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