ID | 116297 |
Author |
Kajiura, Hiroyuki
Osaka University|Hitachi Zosen Corporation|Ritsumeikan University
Yoshizawa, Takuya
Ritsumeikan University
Tokumoto, Yuji
Hitachi Zosen Corporation|University of Zurich
Suzuki, Nobuaki
Hitachi Zosen Corporation
Takeno, Shinya
Hitachi Zosen Corporation
Takeno, Kanokwan Jumtee
Hitachi Zosen Corporation
Yamashita, Takuya
Ritsumeikan University
Tanaka, Shun-ichi
Ritsumeikan University
Kaneko, Yoshinobu
Osaka University
Fujiyama, Kazuhito
Osaka University
Matsumura, Hiroyoshi
Ritsumeikan University
Nakazawa, Yoshihisa
Hitachi Zosen Corporation|Tokushima University
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Content Type |
Journal Article
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Description | Some plant trans-1,4-prenyltransferases (TPTs) produce ultrahigh molecular weight trans-1,4-polyisoprene (TPI) with a molecular weight of over 1.0 million. Although plant-derived TPI has been utilized in various industries, its biosynthesis and physiological function(s) are unclear. Here, we identified three novel Eucommia ulmoides TPT isoforms—EuTPT1, 3, and 5, which synthesized TPI in vitro without other components. Crystal structure analysis of EuTPT3 revealed a dimeric architecture with a central hydrophobic tunnel. Mutation of Cys94 and Ala95 on the central hydrophobic tunnel no longer synthesizd TPI, indicating that Cys94 and Ala95 were essential for forming the dimeric architecture of ultralong-chain TPTs and TPI biosynthesis. A spatiotemporal analysis of the physiological function of TPI in E. ulmoides suggested that it is involved in seed development and maturation. Thus, our analysis provides functional and mechanistic insights into TPI biosynthesis and uncovers biological roles of TPI in plants.
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Journal Title |
Communications Biology
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ISSN | 23993642
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Publisher | Springer Nature
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Volume | 4
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Start Page | 215
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Published Date | 2021-02-16
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Rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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language |
eng
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departments |
Bioscience and Bioindustry
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