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ID 116297
Author
Kajiura, Hiroyuki Osaka University|Hitachi Zosen Corporation|Ritsumeikan University
Yoshizawa, Takuya Ritsumeikan University
Tokumoto, Yuji Hitachi Zosen Corporation|University of Zurich
Suzuki, Nobuaki Hitachi Zosen Corporation
Takeno, Shinya Hitachi Zosen Corporation
Takeno, Kanokwan Jumtee Hitachi Zosen Corporation
Yamashita, Takuya Ritsumeikan University
Tanaka, Shun-ichi Ritsumeikan University
Kaneko, Yoshinobu Osaka University
Fujiyama, Kazuhito Osaka University
Matsumura, Hiroyoshi Ritsumeikan University
Content Type
Journal Article
Description
Some plant trans-1,4-prenyltransferases (TPTs) produce ultrahigh molecular weight trans-1,4-polyisoprene (TPI) with a molecular weight of over 1.0 million. Although plant-derived TPI has been utilized in various industries, its biosynthesis and physiological function(s) are unclear. Here, we identified three novel Eucommia ulmoides TPT isoforms—EuTPT1, 3, and 5, which synthesized TPI in vitro without other components. Crystal structure analysis of EuTPT3 revealed a dimeric architecture with a central hydrophobic tunnel. Mutation of Cys94 and Ala95 on the central hydrophobic tunnel no longer synthesizd TPI, indicating that Cys94 and Ala95 were essential for forming the dimeric architecture of ultralong-chain TPTs and TPI biosynthesis. A spatiotemporal analysis of the physiological function of TPI in E. ulmoides suggested that it is involved in seed development and maturation. Thus, our analysis provides functional and mechanistic insights into TPI biosynthesis and uncovers biological roles of TPI in plants.
Journal Title
Communications Biology
ISSN
23993642
Publisher
Springer Nature
Volume
4
Start Page
215
Published Date
2021-02-16
Rights
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
cb_4_215.pdf 3.05 MB
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry