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ID 116671
Author
Ichihara, Aina Okayama University
Ojima, Hinako Okayama University
Gotoh, Kazuyoshi Okayama University
Matsushita, Osamu Okayama University
Take, Susumu Kurashiki Central Hospital
Okada, Hiroyuki Okayama University
Yokota, Kenji Okayama University
Keywords
antibody
VacA
CagA
genome
Content Type
Journal Article
Description
The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with different reactivity. The search was performed on the common genes, with the homology analysis conducted using a genome ring and dot plot analysis. The two antigens of the highly reactive strains showed a high gene homology, and Western blots for CagA and VacA also showed high expression levels of proteins. In the poorly responsive antigen strains, it was found that the inversion occurred around the vacA gene in the genome. The structure of bacterial genomes might contribute to the poor reactivity exhibited by the antibodies of patients. In the future, an accurate serodiagnosis could be performed by using a strain with few gene mutations of the antigen used for the antibody titer test of H. pylori.
Journal Title
Toxins
ISSN
20726651
Publisher
MDPI
Volume
13
Issue
7
Start Page
467
Published Date
2021-07-05
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences