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ID 117784
Title Alternative
Application of Pore-forming Toxin as a DDS Tool
Author
Ohkubo, Yukimasa The University of Tokushima
Sakakura, Eriko The University of Tokushima
Ohkura, Kazuto Suzuka University of Medical Science
Keywords
Cholesterol-dependent cytolysin
drug-delivery system
chimera toxin
Content Type
Journal Article
Description
Background/Aim: Cholesterol-dependent cytolysins (CDCs) are pore-forming toxins from Gram-positive bacteria. The aim of this study was to investigate the potential of a CDC, intermedilysin, as a drug-delivery system (DDS) for clinical application. Materials and Methods: Intermedilysin was modified by the addition of a disulfide bridge to regulate pore formation, by swapping domain 4 to provide cholesterol-binding capacity, and by the introduction of a targeting domain. The resultant chimera protein, His-LTBP-CDC(ss)IP, was investigated for its use as a DDS tool in vitro. Results: His-LTBP-CDC(ss)IP exhibited a regulated pore-forming capacity under reducing conditions. This chimera protein was able to deliver a drug-carrier liposome specifically to the target cell, to be endocytosed into the cell with subsequent release of the components into the cytoplasm. Conclusion: A chimera protein derived from the bacterial pore-forming toxin intermedilysin (His-LTBP-CDC(ss)IP) forms the basis for a novel DDS tool.
Journal Title
Anticancer Research
ISSN
02507005
17917530
NCID
AA10625860
AA12440673
Publisher
The International Institute of Anticancer Research
Volume
32
Issue
6
Start Page
2323
End Page
2329
Published Date
2012-05-28
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry