ID | 117793 |
Title Alternative | Fine structure of OPCs observed by SBF-SEM
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Author |
Ono, Katsuhiko
Kyoto Prefectural University of Medicine
Gotoh, Hitoshi
Kyoto Prefectural University of Medicine
Nomura, Tadashi
Kyoto Prefectural University of Medicine
Morita, Tsuyoshi
Tokushima University
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Baba, Otto
Tokushima University
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Matsumoto, Mami
National Institute for Physiological Sciences|Nagoya City University
Saitoh, Sei
National Institute for Physiological Sciences|Fujita Health University
Ohno, Nobuhiko
Jichi Medical University|National Institute for Physiological Sciences
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Content Type |
Journal Article
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Description | Oligodendrocyte precursor cells (OPC) arise from restricted regions of the central nervous system (CNS) and differentiate into myelin-forming cells after migration, but their ultrastructural characteristics have not been fully elucidated. This study examined the three-dimensional ultrastructure of OPCs in comparison with other glial cells in the early postnatal optic nerve by serial block-face scanning electron microscopy. We examined 70 putative OPCs (pOPC) that were distinct from other glial cells according to established morphological criteria. The pOPCs were unipolar in shape with relatively few processes, and their Golgi apparatus were localized in the perinuclear region with a single cisterna. Astrocytes abundant in the optic nerve were distinct from pOPCs and had a greater number of processes and more complicated Golgi apparatus morphology. All pOPCs and astrocytes contained a pair of centrioles (basal bodies). Among them, 45% of pOPCs extended a short cilium, and 20% of pOPCs had centrioles accompanied by vesicles, whereas all astrocytes with basal bodies had cilia with invaginated ciliary pockets. These results suggest that the fine structures of pOPCs during the developing and immature stages may account for their distinct behavior. Additionally, the vesicular transport of the centrioles, along with a short cilium length, suggests active ciliogenesis in pOPCs.
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Journal Title |
PLOS ONE
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ISSN | 19326203
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Publisher | PLOS
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Volume | 17
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Issue | 12
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Start Page | e0278118
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Published Date | 2022-12-01
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Rights | This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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language |
eng
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Publisher
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departments |
Oral Sciences
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