ID | 118072 |
Title Alternative | SUDACHITIN IS A SAFE ANTICANCER ADJUVANT TARGETING THE TME
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Author |
Chen, Shuhai
Tokushima University
Nishi, Masaaki
Tokushima University
Tokushima University Educator and Researcher Directory
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Morine, Yuji
Tokushima University
Tokushima University Educator and Researcher Directory
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Tokunaga, Takuya
Tokushima University
Tokushima University Educator and Researcher Directory
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Kashihara, Hideya
Tokushima University
Tokushima University Educator and Researcher Directory
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Takasu, Chie
Tokushima University
Tokushima University Educator and Researcher Directory
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Wada, Yuma
Tokushima University
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Nakamoto, Akiko
Tokushima University
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Sakai, Tohru
Tokushima University
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Keywords | cancer‑associated fibroblasts
sudachitin
glycolysis
reverse Warburg effect
antitumor
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Content Type |
Journal Article
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Description | Sudachitin is a polymethoxylated flavone found in the peel of Citrus sudachi, a unique specialty citrus fruit in Tokushima Prefecture, Japan. Previous reports have demonstrated that sudachitin has anti‑inflammatory and metabolic regulatory activities. However, to the best of our knowledge, no studies have explored whether sudachitin can act as an antitumor therapeutic agent by regulating metabolic functions in the tumor microenvironment. In the present study, cell proliferation and cytotoxicity assays were used to determine whether sudachitin inhibited the in vitro growth of liver cancer and pancreatic carcinoma, cholangiocarcinoma and colorectal cancer cells and to compare its toxicity against normal fibroblasts and induced cancer‑associated fibroblasts (CAFs). Using lactate assays and reverse transcription‑quantitative PCR, the effects of sudachitin on glycolysis in CAFs were investigated. The effects of CAFs on malignant tumor cells were evaluated in vitro using cell proliferation, wound healing and invasion assays. As result, sudachitin inhibited various types of tumor cells with different half‑maximal inhibitory concentrations. Treatment with 50 µM sudachitin for 48 h suppressed tumor and CAFs proliferation but was not cytotoxic against normal fibroblasts. This dose also inhibited glycolysis in CAFs and, thus, diminished their pro‑tumorigenic activities. Overall, the present study revealed that sudachitin has promise as a safe and widely available natural antitumor adjuvant.
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Journal Title |
Oncology Letters
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ISSN | 17921074
17921082
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Publisher | Spandidos Publications
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Volume | 24
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Issue | 1
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Start Page | 236
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Published Date | 2022-05-31
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Rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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DOI (Published Version) | |
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language |
eng
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TextVersion |
Publisher
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departments |
Medical Sciences
University Hospital
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