ID | 118878 |
Title Alternative | 脳梗塞モデルラットにおける虚血後の時期依存的な抗炎症性M2マクロファージ活性化変調の役割
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Author |
Kurashiki, Yoshitaka
Tokushima University
Kinouchi, Tomoya
Tokushima University
Sumiyoshi, Manabu
Tokushima University
Kitazato, Keiko T
Tokushima University
Uto, Yoshihiro
Tokushima University
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Keywords | Arginase-1
cerebral ischemia
interleukin-4
macrophage modulation
post-ischemic phase
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Content Type |
Thesis or Dissertation
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Description | Cerebral ischemia triggers inflammatory changes, and early complications and unfavorable outcomes of endovascular thrombectomy for brain occlusion promote the recruitment of various cell types to the ischemic area. Although anti-inflammatory M2-type macrophages are thought to exert protective effects against cerebral ischemia, little has been clarified regarding the significance of post-ischemic phase-dependent modulation of M2-type macrophages. To test our hypothesis that post-ischemic phase-dependent modulation of macrophages represents a potential therapy against ischemic brain damage, the effects on rats of an M2-type macrophage-specific activator, Gc-protein macrophage-activating factor (GcMAF), were compared with vehicle-treated control rats in the acute (day 0–6) or subacute (day 7–13) phase after ischemia induction. Acute-phase GcMAF treatment augmented both anti-inflammatory CD163+M2-type- and pro-inflammatory CD16+ M1-type macrophages, resulting in no beneficial effects. Conversely, subacute-phase GcMAF injection increased only CD163+ M2-type macrophages accompanied by elevated mRNA levels of arginase-1 and interleukin-4. M2-type macrophages co-localized with CD36+ phagocytic cells led to clearance of the infarct area, which were abrogated by clodronate-liposomes. Expression of survival-related molecules on day 28 at the infarct border was augmented by GcMAF. These data provide new and important insights into the significance of M2-type macrophage-specific activation as post-ischemic phase-dependent therapy.
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Journal Title |
Journal of Cerebral Blood Flow & Metabolism
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ISSN | 15597016
0271678X
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NCID | AA12510484
AA10458430
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Publisher | International Society for Cerebral Blood Flow and Metabolism|Sage
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Volume | 43
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Issue | 4
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Start Page | 531
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End Page | 541
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Published Date | 2022-12-22
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Yoshitaka Kurashikiの学位論文として提出され,学位審査・授与の対象となっている。 |
Rights | Kurashiki Y, Kagusa H, Yagi K, et al. Role of post-ischemic phase-dependent modulation of anti-inflammatory M2-type macrophages against rat brain damage. Journal of Cerebral Blood Flow & Metabolism. 2023;43(4):531-541. Copyright © 2022 The Author(s). doi:10.1177/0271678X221147090
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3766号
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Diploma Number | 甲医第1591号
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Granted Date | 2023-12-28
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
University Hospital
Medical Sciences
Bioscience and Bioindustry
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