ID | 118912 |
Author |
Sasaki, Risa
Kwansei Gakuin University
Toda, Shogo
Kwansei Gakuin University
Sakamoto, Takaiku
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Sakuradani, Eiji
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Shigeto, Shinsuke
Kwansei Gakuin University
|
Content Type |
Journal Article
|
Description | Thraustochytrids are heterotrophic marine protists known for their high production capacity of various compounds with health benefits, such as polyunsaturated fatty acids and carotenoids. Although much effort has been focused on developing optimal cultivation methods for efficient microbial production, these high-value compounds and their interrelationships are not well understood at the single-cell level. Here we used spontaneous (linear) Raman and multiplex coherent anti-Stokes Raman scattering (CARS) micro-spectroscopy to visualize and characterize lipids (e.g., docosahexaenoic acid) and carotenoids (e.g., astaxanthin) accumulated in single living Aurantiochytrium limacinum cells. Spontaneous Raman imaging with the help of multivariate curve resolution−alternating least-squares enabled us to make unambiguous assignments of the molecular components we detected and derive their intracellular distributions separately. Near-IR excited CARS imaging yielded the Raman images at least an order of magnitude faster than spontaneous Raman imaging, with suppressed contributions of carotenoids. As the culture time increased from 2 to 5 days, the lipid amount increased by a factor of ∼7, whereas the carotenoid amount did not change significantly. Furthermore, we observed a highly localized component in A. limacinum cells. This component was found to be mixed crystals of guanine and other purine derivatives. The present study demonstrates the potential of the linear−nonlinear Raman hybrid approach that allows for accurate molecular identification and fast imaging in a label-free manner to link information derived from single cells with strategies for mass culture of useful thraustochytrids.
|
Journal Title |
The Journal of Physical Chemistry B
|
ISSN | 15206106
15205207
|
NCID | AA11114073
AA12189704
|
Publisher | ACS Publications
|
Volume | 127
|
Issue | 12
|
Start Page | 2708
|
End Page | 2718
|
Published Date | 2023-03-15
|
Rights | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
|
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
|
TextVersion |
Publisher
|
departments |
Bioscience and Bioindustry
|