ID 25668
Author
Hagi, Akifumi Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
Hayashi, Hideki Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
Kishi, Kazuhiro Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
Lihong, Wang Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima
Ebina, Yousuke Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
fMLP
PAF
glucose transport
GLUT1
G-protein coupled receptor
Content Type
Journal Article
Description
The chemoattractants, fMLP and PAF, stimulate glucose uptake in phagocytes to obtain an energy source for host defense. Glucose uptake in phagocytes is mainly regulated via glucose transporter type1 (GLUT1). To examine molecular mechanisms of facilitated glucose uptake in response to fMLP or PAF, we established CHO cells stably expressing fMLP or PAF receptor with c-myc epitope tagged GLUT1 which could immunologically detect GLUT1 on the cell surface. In the CHO cells, both fMLP and PAF directly triggered GLUT1 translocation from the intracellular pool to the cell surface, and stimulated glucose uptake. Therefore, in phagocytes, we propose that fMLP and PAF also trigger GLUT1 translocation to stimulate glucose uptake as an energy source for host defense.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
47
Issue
1-2
Start Page
19
End Page
28
Sort Key
19
Published Date
2000
Remark
EDB ID
FullText File
language
eng
departments
Institute of Advanced Medical Sciences